Journal: Cancers

Article Title: Association of Proteasome Activity and Pool Heterogeneity with Markers Determining the Molecular Subtypes of Breast Cancer

doi: 10.3390/cancers17010159

Figure Lengend Snippet: Proteasome activities and content in BC and adjacent tissues. Representative immunohistochemical staining of tumor tissues ( a ). First row ( A1 – A4 ) Invasive carcinoma of nonspecific type (Luminal A subtype): A1 —Strong expression of ER; A2 —Strong expression of PR; A3 —negative expression of HER-2; A4 —low level of Ki-67. Second row ( B1 – B4 ) Invasive carcinoma of nonspecific type (Luminal B subtype): B1 —Strong expression of ER; B2 —Strong expression of PR; B3 —weak (1+) expression of HER-2; A4 —high level of Ki-67 (>20%). Third row ( C1 – C4 ) Invasive carcinoma of nonspecific type (Triple negative subtype): C1 —negative expression of ER; C2 , negative expression of PR; C3 , negative expression of HER-2; A4 , high level of Ki-67 (>50%). Immunohistochemistry, ×200. Scale bar—100 μm. ( b ) ChTL and CL proteasome activities in BC and adjacent tissues ( c ). Proteasome activities in tissues of various molecular subtypes of BC. ( d ) The content of 20S proteasome or proteasome regulator subunits ((α1, α2, α3, α5, α6, α7), Rpt6, PA28β LMP-2, LMP-7) determined by Western blotting in BC (1) and adjacent tissues (2). Representative Western blots are shown. ( e ) Content of proteasome subunits in tumour and adjacent tissue; and in different molecular subtypes of the BC ( f ) determined via quantification of blots using the ImageJ software ver. 1.54i. Red box indicates the relative content of proteasome subunits in the adjacent tissue, which was considered as 100%; * p < 0.05, ** p < 0.01, Mann-Whitney test.

Article Snippet: Primary mouse monoclonal antibodies to 20S proteasome subunits α1, α2, α3, α5, α6, α7 (cat. # sc-58412), mouse mAbs to LMP-7 (cat. # sc-100284), rabbit polyclonal antibodies (pAbs) to LMP-2 (cat. # sc-28809), and goat antibodies to the subunit PA28β (cat. # sc-23642) were obtained from Santa Cruz Biotechnology, Dallas, TX, USA.

Techniques: Immunohistochemical staining, Staining, Expressing, Immunohistochemistry, Western Blot, Software, MANN-WHITNEY

Journal: Cancers

Article Title: Association of Proteasome Activity and Pool Heterogeneity with Markers Determining the Molecular Subtypes of Breast Cancer

doi: 10.3390/cancers17010159

Figure Lengend Snippet: Relative content of proteasome subunits in tumor tissue in different molecular subtypes of BC.

Article Snippet: Primary mouse monoclonal antibodies to 20S proteasome subunits α1, α2, α3, α5, α6, α7 (cat. # sc-58412), mouse mAbs to LMP-7 (cat. # sc-100284), rabbit polyclonal antibodies (pAbs) to LMP-2 (cat. # sc-28809), and goat antibodies to the subunit PA28β (cat. # sc-23642) were obtained from Santa Cruz Biotechnology, Dallas, TX, USA.

Techniques:

Journal: Cancers

Article Title: Association of Proteasome Activity and Pool Heterogeneity with Markers Determining the Molecular Subtypes of Breast Cancer

doi: 10.3390/cancers17010159

Figure Lengend Snippet: Correlations between proteasome activities, expression of estrogen and progesterone receptors, Ki-67 and HER-2 receptor.

Article Snippet: Primary mouse monoclonal antibodies to 20S proteasome subunits α1, α2, α3, α5, α6, α7 (cat. # sc-58412), mouse mAbs to LMP-7 (cat. # sc-100284), rabbit polyclonal antibodies (pAbs) to LMP-2 (cat. # sc-28809), and goat antibodies to the subunit PA28β (cat. # sc-23642) were obtained from Santa Cruz Biotechnology, Dallas, TX, USA.

Techniques: Expressing

Journal: Cancers

Article Title: Association of Proteasome Activity and Pool Heterogeneity with Markers Determining the Molecular Subtypes of Breast Cancer

doi: 10.3390/cancers17010159

Figure Lengend Snippet: Interrelation and mutual influence of proteasome activities and indicators on the basis of which the molecular subtype of breast cancer is determined. Note: → The positive influence of one characteristic on another.

Article Snippet: Primary mouse monoclonal antibodies to 20S proteasome subunits α1, α2, α3, α5, α6, α7 (cat. # sc-58412), mouse mAbs to LMP-7 (cat. # sc-100284), rabbit polyclonal antibodies (pAbs) to LMP-2 (cat. # sc-28809), and goat antibodies to the subunit PA28β (cat. # sc-23642) were obtained from Santa Cruz Biotechnology, Dallas, TX, USA.

Techniques: